Fig. 2From: The impact of neutrophil extracellular trap from patients with systemic lupus erythematosus on the viability, CD11b expression and oxidative burst of healthy neutrophilsThe Detection of dead cells by flow cytometry. The blood granulocytes isolated were cultured with the NET of either the healthy subjects or the patients for about 4 h, then stained with FITC-annexin-V and PI to differentiate between the viable, necrotic, early and late apoptotic cells. Plots B and C represent the same sample double-stained after coculture with normal (b) and SLE neutrophil-derived NET (c); plot A shows the same sample unstained. An-V: annexin-V, PI: Propidium IodideBack to article page