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Fig. 2 | BMC Immunology

Fig. 2

From: The impact of neutrophil extracellular trap from patients with systemic lupus erythematosus on the viability, CD11b expression and oxidative burst of healthy neutrophils

Fig. 2

The Detection of dead cells by flow cytometry. The blood granulocytes isolated were cultured with the NET of either the healthy subjects or the patients for about 4 h, then stained with FITC-annexin-V and PI to differentiate between the viable, necrotic, early and late apoptotic cells. Plots B and C represent the same sample double-stained after coculture with normal (b) and SLE neutrophil-derived NET (c); plot A shows the same sample unstained. An-V: annexin-V, PI: Propidium Iodide

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