Skip to main content
Fig. 3 | BMC Immunology

Fig. 3

From: The impact of neutrophil extracellular trap from patients with systemic lupus erythematosus on the viability, CD11b expression and oxidative burst of healthy neutrophils

Fig. 3

The effects of NET on the death of granulocytes. The death of neutrophils was evaluated after 4 h-contact with the NET of both healthy subjects and patients. In the case of early apoptosis, no significant differences were observed between the coculture with NET, NCM or nothing. Regarding late apoptosis, a significant increase was detected in the cocultures with either normal or SLE NET in comparison to the control cocultures (the cocultures contained no NET or NCM). The level of late apoptosis was also significantly higher in the cocultures of SLE NET than the cocultures of normal NET. The percentage of necrotic neutrophils after contact with the NET of both healthy subjects and patients for about 4 h increased significantly compared to the neutrophils in contact with no NET. The difference in the percentage of necrotic cells was statistically insignificant between the SLE NET-contained cocultures and the normal NET-contained cocultures. NCM: NET control medium, which refers to the medium that was collected from the unstimulated healthy and SLE neutrophils in the NET-inducing experiments

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us