Fig. 1

Flow chart of high-throughput immunoglobulin protein quantification by plasma microarray. Plasma samples from HCs and BD patients with or without corticosteroid and immunosuppressant treatment were printed on a microscope slide using an arrayer together with different concentrations of purified immunoglobulin standards. The detection of immunoglobulin in plasma was performed using fluorescein-labeled detection antibody, and the signal was read with a microarray fluorescent scanner at wavelengths of 532 nm and 635 nm, separately. The quantified immunoglobulin proteome was analyzed with 29 clinical variables for their correlations in BD patients without and with appropriate treatments. HC: healthy control; BD: Behcet’s disease