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Table 5 MTT cytotoxicity assay of neutralizing activity of anti-trastuzumab Ab developed in patient’s serum using MCF-7 cell line

From: Trastuzumab immunogenicity development in patients’ sera and in laboratory animals

Serum dilutions

% Cytotoxicity

Control

1st Regimen

2nd Regimen

3rd Regimen

 

Value

±SD

Value

±SD

Value

±SD

Value

±SD

0

36.89

0.08

36.89

0.08

36.89

0.08

36.89

0.08

1:20

  

49.19

0.12

29.88

0.05

34.19

0.04

1:100

  

12.12

0.27

1.49

0.12

25.98

0.10

1:200

  

0.76

0.15

13.71

0.02

50.22

0.08

  1. Control: Drug alone added to MCF-7 cell line
  2. 1st Regimen: Both drug and serum dilutions were added simultaneously to MCF-7 cell line
  3. 2nd Regimen: The serum dilutions were added to MCF-7 cell line 1 h before addition of the drug
  4. 3rd Regimen: Both drug and serum dilutions were mixed at equal volumes and left at room temperature for 1 h before their addition to MCF-7 cell line