Fig. 4

Influence of recombinant human CD100 stimulation to CD8⁺ T cells cytotoxicity from HLA-A02 restricted controls (n = 8) and acute myocardial infarction (AMI) patients (n = 10). CD8⁺ T cells were stimulated with recombinant human sCD100 for 24 h. Cells were washed twice, and 10⁴ of stimulated CD8⁺ T cells were co-cultured with 5 × 10⁴ of HUVECs in direct contact and indirect contact co-culture system. Supernatants were harvested 48 h post co-culture. a LDH level in the cultured supernatants were measured using LDH Cytotoxicity Assay Kit. The percentage of HUVECs death was calculated, and was compared between controls and AMI patients, and between presence and absence of CD100 stimulation. b IFN-γ and c TNF-α level in the supernatants was assessed by ELISA, and was compared between controls and AMI patients, and between presence and absence of CD100 stimulation. Individual level for each subject was shown. The horizon lines indicated means, while the error bars indicated standard deviations. One-way ANOVA and SNK-q test was used for statistical analysis