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Table 4 Physical and chemical properties, antigenicity and number of the predicted transmembrane helices of SARS CoV-2 proteins

From: Vaccinomic approach for novel multi epitopes vaccine against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)

Viral protein

Accession number

Molecular weight (Dalton)

Instability indexb

Aliphatic index

Theoretical pI

No amino acids

Extinction coefficient

GRAVYc

Vaxijen antigenicityd

TMHs

orf1ab polyprotein

YP_009724389.1

794,057.79

33.31

86.87

6.32

7096

942,275

−0.07

0.4624

14

orf1a polyprotein

YP_009725295.1

489,988.91

34.92

88.99

6.04

4405

552,175

−0.023

0.4787

14

Surface glycoprotein

YP_009724390.1

141,178.47

33.01

84.67

6.24

1273

148,960

−0.079

0.4646

1

orf3a protein

YP_009724391.1

31,122.94

32.96

103.42

5.55

275

58,705

0.275

0.4945

3

Envelope protein

YP_009724392.1

8365.04

38.68

144

8.57

75

6085

1.128

0.6025

1

Membrane glycoprotein

YP_009724393.1

25,146.62

39.14

120.86

9.51

222

52,160

0.446

0.5102

3

orf6 protein

YP_009724394.1

7272.54

31.16

130.98

4.6

61

8480

0.233

0.6131

0

aorf7a protein

YP_009724395.1

13,744.17

48.66

100.74

8.23

121

7825

0.318

0.6441

1

orf7b protein

YP_009725296.1

5180.27

50.96

156.51

4.17

43

7115

1.449

0.6025

1

orf8 protein

YP_009724396.1

13,831.01

45.79

97.36

5.42

121

16,305

0.219

0.6502

0

Nucleocapsid phosphoprotein

YP_009724397.2

45,625.7

55.09

52.53

10.07

419

43,890

−0.971

0.5059

0

aorf10 protein

YP_009725255.1

4449.23

16.06

107.63

7.93

38

4470

0.637

0.7185

0

  1. THMs Transmembrane helices
  2. athe protein contains no tryptophan
  3. binstability index < 40 considered the protein stable
  4. cGRAVY negative sign indicated the protein is hydrophilic
  5. dthe threshold for the Vaxijen antigenicity is 0.4