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Fig. 1 | BMC Immunology

Fig. 1

From: A putative exosporium lipoprotein GBAA0190 of Bacillus anthracis as a potential anthrax vaccine candidate

Fig. 1

GBAA0190 protein expression in the surface of B. anthracis spore. a The Nucleotide and amino acid sequences of GBAA0190. The positively charged region and lipobox were shown in bold, whereas the positively charged region and cysteine residue were underlined. b GBAA0190 protein was specifically expressed in B. anthracis spores. Protein fractions such as spore lysates (SL, 30 μg), vegetative cell proteins (VCP, 30 μg), and secreted proteins (SP, 30 μg) of B. anthracis H9401 and r0190 (1 μg) were analyzed by SDS-PAGE, followed by staining with coomassie brilliant blue (CBB) and Western blotting with normal mouse serum or r0190 immune serum (1: 1000 dilution). c Detection of GBAA0190 protein on B. anthracis H9401 spore exosporium. Spores were treated with fixation solution and incubated with r0190 immune serum (1:1000 dilution), followed by incubation with 10 nm gold-conjugated secondary antibodies (black dots)

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