Fig. 3

Involvement of TLR2 and TLR1 in r0190-induced inflammatory response. BMDMs from C57BL/6 and TLR2-KO C57BL/6 mice were stimulated with r0190 (0.1, 0.3, or 1 μg/ml), P3C (0.5 μg/ml), or CpG 2395 (0.1 μΜ) for 24 h. The level of TNF-α (a) and IL-6 (b) were measured by ELISA. c HEK293-TLR2 cells were pretreated with control IgG1 (5 μg/ml), α-TLR1 (5 μg/ml), or α-TLR6 (5 μg/ml) for 1 h, followed by stimulation with r0190 (1 μg/ml), P3C (0.1 μg/ml), or MALP2 (0.01 μg/ml) for an additional 24 h. Cell culture media were then collected and IL-8 concentration was measured by ELISA. .* P < 0.05, ** P < 0.01 as compared with the appropriate control group