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Fig. 4 | BMC Immunology

Fig. 4

From: Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation

Fig. 4

MyD88-dependent and independent MRs are significantly enriched in biological processes related to inflammation and chemotaxis. a Comparison of Bb internalization by WT BMDMs (black bars) at MOI 10:1 with MyD88−/− BMDMs (red bars) at MOI 100:1. b Number of differentially expressed genes (DEGs) in Bb-infected WT and MyD88−/− BMDMs determined by RNA-sequencing. Red bar indicates number of upregulated DEGs and blue bar indicates number of down-regulated DEGs. Bar height represents total number of DEGs in each condition. c Venn diagram depicting DEG classification. MyD88-dependent genes (light gray, left) are only differentially expressed in WT BMDMs. MyD88-independent genes (center) are expressed in both cell types. MyD88-privative genes (dark gray, right) are only differentially expressed in MyD88−/− BMDMs. d Venn diagram comparing biological processes (BP) relating to inflammation significantly enriched between MyD88-dependent (light gray) and MyD88-privative (dark gray) master regulators. e Heat map showing fold change of master regulators enriched in inflammation in WT (cyan) or MyD88−/− (yellow) BMDMs. GO numbers for significantly enriched BP are indicated on the x-axis. Heat maps were generated using R statistical software, package “heatmap2”

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