Fig. 5

Adoptive bone marrow transplantation of TREM2 TG mice increase NK cell population in WT mice. a Representative flow cytometry plots of CD3⁻NK1.1⁺cells from spleen, BM, and lung of lethally irradiated WT CD45.1 recipient 4 weeks after tail vein injection of total BM from WT or TREM2-TG (CD45.2) donors. The percentage (left) of CD45.2 + NK1.1+ cells in the spleen, BM, and lung of WT CD45.1 recipient (N = 4). Each symbol represents an individual mouse; horizontal lines indicate the mean. b Graph depicting tumor volumes in tumor-bearing CD45.1 WT mice after intravenous injection with BM cells of CD45.2 WT (open square, N = 4) or CD45.2 TREM2-TG mice (solid circle, N = 4). Tumor volume was measured every 2 days, after 15 days of injection. Significance of difference was analyzed by two-way ANOVA analysis with Bonferroni posthoc test (*P < 0.05). Three independent experiments repeated. c The survival rate of tumor-bearing CD45.1 WT mice after intravenous injection with BM cells from CD45.2 WT (open square, N = 4) or CD45.2 TREM2-TG mice (solid circle, N = 4). *P < 0.05. d Representative photographs of lungs with metastatic colonies (top panel) of tumor-bearing CD45.1 WT mice after intravenous injection with BM cells of CD45.2 WT or CD45.2 TREM2-TG mice. Graph quantifies the total number of metastatic colonies in the lung (bottom panel). **P < 0.01 using Student’s t-test