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Fig. 6 | BMC Immunology

Fig. 6

From: Direct effects of mast cell proteases, tryptase and chymase, on bronchial epithelial integrity proteins and anti-viral responses

Fig. 6

Blocking of protease activity abolish the Poly (I:C)-impairment of IFN and PRR expression by tryptase. Tryptase or chymase were pre-incubate with AEBSF, then pre-treated with tryptase or chymase alone or in combination with AEBSF followed by stimulation with Poly (I:C). Gene expression was measured in cell lysate. Gene expression of IFN-β (a), IFN-lambda (b), TLR3 (c), MDA-5 (d) and RIG-I (e). Protein expression of TLR3 (f), MDA-5 (g) and RIG-I (h). Representative western blot of PRR protein expression at 24 h (i). Data are presented as mean ± SEM, n = 4 from 4 independent experiments, *P < 0.05 compared to respective control, #P < 0.05 compared to Poly (I:C) alone and &P < 0.05 compared to combination with tryptase and Poly (I:C) and AEBSF

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