Fig. 3

Spontaneous anti-tumor and antiviral responses before and after CRT. A PBMCs from 26 patients treated with CRT for a lung cancer or a head and neck cancer were collected before CRT and 1 month after. After short stimulation (1 week) with a mixture of HLA class II peptides derived from TERT or viral peptides, the presence of TERT-specific T cells was detected by IFNγ ELISPOT assay. The results represented specific IFNγ spots after subtraction of background. Responses were positive when IFNγ spots were more than 10 and more than 2-fold the background. B Response against TERT in a representative patient with loss of anti-TERT Th1 reponse. Bottom: histograms represented specific IFNγ spots number in medium (grey) and TERT (black). Top: illustration of medium and TERT ELISPOT wells. C individual variation of the intensity of anti-TERT Th1 response in patients with available data at baseline and 1 month after CRT (n = 19). Lines in red represent significant decrease (> 20%) from baseline. D intensity of specific anti-viral response in 10 patients with available data at baseline. Number of patients with anti-TERT response is shown between brackets