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Fig. 2 | BMC Immunology

Fig. 2

From: Downregulation of NKG2DLs by TGF-β in human lung cancer cells

Fig. 2

Restored surface expression levels of NKG2DLs by blocking of TGF-β signaling. The surface expression levels of NKG2DLs were analyzed using flow cytometry in (A) NCI-H23, (B) SW-900 and (C) A549 lung cancer cells. The lung cancer cells were treated with TGF-β (10 ng/ml) and followed by Galunisertib (10 μM, 20 μM) for 24 h. The relative expression ratios were calculated from mean fluorescence intensities (MFI) of treated cells compared to those of control cells. Filled gray, dotted gray, dark gray, light gray and black lines represent isotype, media control, TGF-β 10 ng/ml treatment, TGF-β 10 ng/ml with Galunisertib 10 μM treatment and TGF-β 10 ng/ml with Galunisertib 20 μM treatment, respectively. The experiments were performed three times. (p < 0.05, *; p < 0.01, **; p < 0.001, ***)

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