Fig. 4

The transcriptions of MMPs were regulated by treatment with TGF-β and Galunisertib. The transcription levels of MMPs were analyzed in (A) NCI-H23, (B) SW-900 and (C) A549 lung cancer cells by multiplex RT-PCR. Lung cancer cells were treated with TGF-β (10 and 20 ng/ml) and Galunisertib (5 and 10 μM) for 18 h. The PCR products were separated and quantified by MultiNA. All experiments were done in triplicate. The changes of transcripts were compared to media treated groups after treatment with TGF-β without Galunisertib. The changes of transcription levels were compared to TGF-β treated groups after combined treatment with TGF-β with Galunisertib. The experiments were performed three times. (*# P < 0.05, **## P < 0.01, ***### P < 0.001)