Fig. 5

The level of MMP2 was modulated by treatment with TGF-β and Galunisertib. The MMP2 at protein level were analyzed in (A) NCI-H23, (B) SW-900 and (C) A549 lung cancer cells by western blot. Lung cancer cells were treated with TGF-β (10 and 20 ng/ml) and Galunisertib (5 and 10 μM) for 18 h. Bands were cropped from original images (Fig. S4) and intensity was measured using the ImageJ software and the expression levels were calculated by ratio against β-actin. The experiments were performed three times and showed representative data