Fig. 2

Binding Affinity of Predicted Epitope Peptides to Target Genes (HHLA1,2 & 3 and MAGEB5) by HLA-C Immune variants: Each Immune variant was used at the NetMHCpan 4.0 Server to predict epitope peptides for each protein variant of the target genes. The plotted graphs showed gene-protein variants on the x-axis against and their different predicted epitope peptides represented by their peptide binding score on the y-axis and measured by their binding affinity; 1-log (IC50). A This figure shows the number of protein variants for HHLA1 (n = 2) and number of predicted unique epitope peptides (n = 8). Two epitope peptides [KTLPSTSHW (check) & RRVARTQWL (dot)] were common to both protein variants and one epitope peptide (QASPTSGAF) was predicted for amino acid variations. B This figure shows the number of protein variants for HHLA2 (n = 7) and number of unique predicted epitope peptides (n = 9) with 5 of them (AQTALSFFL, GRWTMKDGL, IQNGNASLF, RGSEVVIHW, YANRTSLFY) repeating in almost all protein variants. C This figure shows the number of protein variants for HHLA3 (n = 5) and number of unique predicted epitope peptides (n = 11) with 7 of them (IISPVTCMY, QRILSQPTF, RRIHRVSLV, TEHLLRAVL, VLSTERGPY, VTCMYTSRW, YPDPKRAFL) repeating in at least two protein variants. D This figure shows the number of protein variants for MAGEB5 (n = 1) and number of unique predicted epitope peptides (n = 4) with one (FVRLTYLEY) repeating due to different binding scores