Fig. 3

Analysis of inflammatory cell infiltration and neuronal cell loss after PBI. The distribution of macrophages and activated microglia was evaluated within the injury site of TSG^+/− and TSG-6^−/− mice 3 days post-injury (dpi) by anti-CD68 immunostaining (red) (A). Neuronal cells were immunostained with anti-β III tubulin (green) in the equivalent area of uninjured TSG^+/− (i) and TSG-6^−/− (ii) mice and within the injury site of TSG-6^−/+ (iii) and TSG-6^−/− (iv) mice 14 days post-injury (dpi). The number of CD68⁺ cells was counted in the injury site and within 100 μm of the wound edge of TSG-6^−/+ and TSG-6^−/− mice 3 days post-injury (C). The relative fluorescent units (RFU) of anti-β III tubulin staining were quantified in and around the injury site of TSG-6^−/+ and TSG-6^−/− mice 14 days post-injury (D). Nuclei were counterstained with DAPI. Scale bar represents 100 μm. * = p ≤ 0.05 comparing TSG-6^−/+ and TSG-6^−/− mice