Table 1 Primers used for real time PCR analysis
Gene (Mus musculus) | Accession number | Forward (5’ → 3’) | Reverse (5’ → 3’) |
|---|---|---|---|
Tenascin C (tnc) | NM_011607.3 | CCAGGGTTGCCACCTATTT | GTCTAGAGGATCCCACTCTACTT |
Gfap | NM_001131020.1 | AACAACCTGGCTGCGTATAG | TCTCGAACTTCCTCCTCATAGAT |
Tsg-6 /Tnfaip6 | NM_009398.2 | CCCACATGCAAAGGAGTGTG | TGAGCCGAATGTGCCAGTAG |
Chst1 | NM_021439.2 | CACCCAGTCATGCGGAGGAA | GCAGGATGGCAGTGTTGGAT |
Chst12 | NM_021528.3 | GAGCTGGAGAACGAAGAGTTT | CAGGAGGTACTGGATGAAGTTG |
IL1β | NM_008361.4 | GTGCAAGTGTCTGAAGCAGC | CTCATCACTGTCAAAAGGTGGC |
Cspg4 | NM_139001.2 | TCTACAGCTCCTGCCTCCTT | ATGTGGAGAACTGGAGCAGC |
Ccl5 (Rantes) | NM_013653.3 | CCTCACCATATGGCTCGGAC | ACGACTGCAAGATTGGAGCA |
Nfkb1 | NM_008689.2 | GTCACCCATGGCACCATAAA | CCTTCACCTTCAGTTTCCTTCTC |
Has1 | NM_008215.2 | CTA TGC TAC CAA GTA TAC CTC G | TCT CGG AAG TAA GAT TTG GAC |
Has2 | NM_008216.3 | CGG TCG TCT CAA ATT CAT CTG | ACA ATG CAT CTT GTT CAG CTC |
Has 3 | NM_008217.4 | GAT GTC CAA ATC CTC AAC AAG | CCC ACT AAT ACA TTG CAC AC |
Itih1 | CCA CCC CAT CGG TTT TGA AGT GTC T | TGC CAC GGG TCC TTG CTG TAG TCT | |
Itih2 | ATG AAA AGA CTC ACG TGC TTT TTC | ATT TGC CTG GGG CCA GT | |
Itih3 | TGA GGA GGT GGC CAA CCC ACT | CGC TTC TCC AGC AGC TGC TC | |
Actb | NM_007393.5 | CACTGTCGAGTCGCGTCC | TCATCCATGGCGAACTGGTG |
Gapdh | NM_001289726.1 | AACAGCAACTCCCACTCTTC | CCTGTTGCTGTAGCCGTATT |