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Fig. 4 | BMC Immunology

Fig. 4

From: The transmembrane domain and luminal C-terminal region independently support invariant chain trimerization and assembly with MHCII into nonamers

Fig. 4

Lack of bidirectional TRIM interactions prevents formation pseudo-heptamers and/or pseudo-pentamers including βSCD. a Schematic representation of DRα and βSCDTRIM. In βSCDTRIM, the IiTRIM and DRβ luminal domains are linked by a flexible gly3/ser/gly3 linker. Top view on the right shows association of an α chain to the βSCDTRIM. b DRα and βSCDTRIM were transiently expressed in HEK293T cells (upper histogram). After 48 h, cells were stained to detect MHCII, Ii and CLIP using L243, 9e10 and CerCLIP.1 mAbs, respectively. Cells were transfected as above, as well as with DM and stained for surface CLIP. A fraction of the cells was permeabilized to detect DM using Map.DM1 mAb (lower histogram). c–e Cells were transfected with DRα and βSCDTRIM alone or together with p35LIML or p35LIMLTRIM. After 48 h, cells were stained to detect surface CLIP (CerCLIP.1) (c). The surface over total expression ratio of MHCII (L243) (d) and Ii (BU45) (e) were calculated. Error bars indicate the SD from five independent experiments. Student t-tests were performed; *p ≤ 0.001

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