Fig. 4

Tregs depletion strengthened the anti-tumor effect of anti-PD-L1 treatment in cervical tumor model. A–D The change of Tregs level in peripheral blood (A), spleen (B), tumor (C) and DLN (D) in the syngeneic tumor model. The above samples were collected at the endpoint. N = 4. Two-tailed unpaired T-test was performed. E The tumor growth curve under different treatment. The tumor was measured with a caliper, and the tumor volumes were calculated with the formula: ½ *length *width*width. Two-tailed unpaired T-test was performed. F The tumor pictures after different treatment. N = 4. G The tumor weight in different groups. n = 4. The tumors were weight with a analytical balance. Two-tailed unpaired T-test was performed. ns: no significant difference, *p < 0.05, **p < 0.01compared to the control groups. A p value less than 0.05 was considered to be statistically significant