Fig. 5

The increased Tregs proliferation depressed the level of effector CD8⁺ T cells after PD-L1 treatment. A The change of Ki67⁺ Tregs in the peripheral blood of mice after anti-PD-L1 treatment. The blood was collected at the endpoint. N = 4. Two-tailed unpaired T-test was performed. B The mRNA level of Ki67 in sorted Tregs cells from the spleen of mouse. N = 3. Two-tailed unpaired T-test was performed. C–E The change of the frequency of effector CD8⁺ T cells in tumor (C), the peripheral blood (D) and DLN (E) at the endpoint. N = 4. The effector CD8 + T cell was defined as CD3⁺CD8⁺CD62L⁻CD44⁺. Two-tailed unpaired T-test was performed. ns: no significant difference, *p < 0.05, **p < 0.01compared to the control groups. A p value less than 0.05 was considered to be statistically significant