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Fig. 2 | BMC Immunology

Fig. 2

From: An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies

Fig. 2

Schematic of the nonstringent TCR cloning method. CD8+ T cells of cancer patients were first cocultured with irradiated autologous PBMC pulsed with the target viral peptide for 7–10 days. The reactivities of T cells were tested by ELISA and T cells with high IFN-γ release were subjected to one round of nonstringent limiting dilution cloning. The second ELISA tests were performed to identify T cells that still had high IFN-γ release. The polyclonal T cells with high activities were expanded in T25 flasks. Tetramer-guided cell sorting was performed to collect 0.5–2 × 106 cells for ImmunoSEQ. The TCR pair was identified by the distribution abundance of TCRα and TCRβ chains

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