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Fig. 7 | BMC Immunology

Fig. 7

From: An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies

Fig. 7

TCR-transduced T cells recognize and kill cancer cells expressing target viral antigens in vitro. a MHC competition assay in which the effector G8 TCR-T cells were cocultured with different target cells (CaSki: HLA-A2+, HPV16-E7+ or SiHa: HLA-A2−, HPV16-E7+) with or without blocking antibodies as indicated in x-axis. UT: untransduced T cells served as a negative control. b LDH release assay of G8 TCR transduced CD4+ and CD8+ T cells. c A6 TCR-T cells were cocultured with glioblastoma cell lines (U87 or T98) expressing CMV antigen or empty vector. d LDH release assay of A6 TCR-transduced T cells against U87 expressing CMV-pp65. The ratio of effector cells to target cells was indicated in the x-axis (E: T ratio). The percentage of cytotoxicity was calculated according to the manufacturers’ instructions

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BMC Immunology

ISSN: 1471-2172

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