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Fig. 3 | BMC Immunology

Fig. 3

From: Activation of Toll-like receptor 2 enhances peripheral and tumor-infiltrating CD8+ T cell cytotoxicity in patients with gastric cancer

Fig. 3

The influence of TLR2 activation on cytotoxic pathways of peripheral and tumor-infiltrating CD8+ T cells from NC and GC patients. CD8+ T cells were purified from peripheral bloods of NC (n = 9) and GC patients (n = 11), as well as from tumor residency of GC patients (n = 11). 2.5 × 104 of CD8+ T cells were cultured for 12 h in the presence of anti-CD3/CD28 with or without TLR2 agonist Pam3Csk4. mRNA relative level corresponding to a perforin, b granzyme B, and c FasL in CD8+ T cells with or without PamCsk4 stimulation was semi-quantified by real-time PCR. The secretion of d perforin and e granzyme B by CD8+ T cells was analyzed by ELISPOT. The columns indicated means, and the bars indicated standard deviation. Significances were determined by LSD-t test or paired t test

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