Fig. 5
The influence of TLR2 activation on cytolytic function of peripheral and tumor-infiltrating CD8+ T cells from NC and GC patients. CD8+ T cells were purified from HLA-A2 restricted individuals, including NC (peripheral bloods, n = 6) and GC patients (peripheral bloods and tumor specimens, n = 17), and were stimulated with or without Pam3Csk4 for 12 h in the presence of anti-CD3/CD28. Cells were washed twice, and 105 of stimulated CD8+ T cells were cocultured in direct and indirect contact with 5 × 105 of AGS cells for 48 h. The percentage of AGS cell death in a direct contact coculture system and b indirect contact coculture system was calculated by measuring lactate dehydrogenase release in the supernatants. The columns indicated means, and the bars indicated standard deviation. Significances were determined by One-Way ANOVA, LSD-t test or paired t test