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Fig. 1 | BMC Immunology

Fig. 1

From: PI3Kδ inhibition prevents IL33, ILC2s and inflammatory eosinophils in persistent airway inflammation

Fig. 1

Female BALB/c mice were sensitised with HDM (25 μg/50 μl) via the intra-nasal route, once a day, 5 days a week over a 3 week period (Days 0–18). Inflammation in the airways was allowed to resolve for a period of 2 weeks (Days 19–33). On day 33, respective groups of mice were sacrificed at the end of the resolution period. Other groups of mice were challenged with either saline or re-challenged with HDM (100 µl/50 µl) and relevant groups were sacrificed at various time-points post re-challenge (4 h–7 days) to quantify; cells within the lung tissue and cells and soluble mediators in broncho-alveolar lavage. For studies involving therapeutics, mice were sacrificed at 72 h post re-challenge to quantify myeloid subsets (eosinophils) or lymphoid subsets (ILC2, CD4 positive subsets and B cells). All HDM sensitisation and re-challenges indicated by red arrows. All terminal endpoints are indicated by green arrows

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