Fig. 2

Characterisation of the cell populations present in the airways at the end of the sensitisation or during and after resolution. A Three weeks of repeated HDM exposure resulted in a granulocytic and lymphocytic infiltration into bronco-alveolar lavage and also B induced an inflammatory mediator response in the airways. C This inflammatory profile was allowed to resolve over a 15 day period. At the end of resolution, eosinophils, lymphocytes and macrophages remained in the broncho-alveolar lavage in significantly increased numbers compared to saline sensitized controls. Neutrophil numbers were not different to saline controls, (n = 4–8 mice per group in saline controls and 6–15 in HDM groups). ***p < 0.001 compared to saline controls. D Cell populations present in lung tissue at the end of the 15 day resolution period. n = 4 mice per naïve or HDM group. Data analysed using one-way analysis of variance (ANOVA) with Dunnets p value adjustment, *p < 0.05, **p < 0.01, ***p < 0.001 compared to naïve unsensitised mice