Natural immunogenic properties of bioinformatically predicted linear B-cell epitopes of dengue envelope and pre-membrane proteins

Nadugala, Mahesha N.; Jeewandara, Chandima; Jadi, Ramesh S.; Malavige, Gathsaurie N.; de Silva, Aravinda M.; Premaratne, Prasad H.; Goonasekara, Charitha L.

doi:10.1186/s12865-021-00462-4

Table 3 The antibody responses of the four representative DENV peptides of E protein epitopes with less than 50% pan serotype conservancy

From: Natural immunogenic properties of bioinformatically predicted linear B-cell epitopes of dengue envelope and pre-membrane proteins

Epitope ID/peptide ID		Serotype of sera				% Sensitivity	% Specificity
Epitope ID/peptide ID		DENV1	DENV2	DENV3	DENV4	% Sensitivity	% Specificity
EP4/E	DENV1	0 0%	0 0%	0 0%	0 0%	0	100
	DENV2	0 0%	0 0%	0 0%	0 0%	0	100
	DENV3	1 8%	0 0%	2 17%	0 0%	17	97.2
	DENV4	0 0%	1 8%	0 0%	4 33%	33	97.2
EP8/E	DENV1	10 83%	2 17%	5 42%	1 8%	83	77.8
	DENV2	5 42%	4 33%	0 0%	2 17%	33	80
	DENV3	8 67%	1 8%	8 67%	0 0%	67	75
	DENV4	0 0%	0 0%	0 0%	0 0%	0	100
EP9/E	DENV1	0 0%	0 0%	0 0%	0 0%	0	100
	DENV2	0 0%	0 0%	0 0%	0 0%	0	100
	DENV3	0 0%	0 0%	0 0%	0 0%	0	100
	DENV4	0 0%	0 0%	0 0%	0 0%	0	100
EP11-12/E	DENV1	10 83%	0 0%	1 8%	0 0%	83	97.2
	DENV2	0 0%	10 83%	0 0%	0 0%	83	100
	DENV3	0 0%	2 17%	5 42%	3 25%	42	86
	DENV4	0 0%	0 0%	4 33%	4 33%	33	89
EP14/E	DENV1	2 17%	0 0%	0 0%	1 8%	17	97.2
	DENV2	2 17%	12 100%	12 100%	0 0%	100	61
	DENV3	0 0%	10 83%	11 92%	0 0%	92	72
	DENV4	0 0%	0 0%	0 0%	11 92%	92	100
EP17/E	DENV1	3 25%	1 8%	4 33%	1 8%	25	83
	DENV2	0 0%	6 50%	0 0%	5 42%	50	86
	DENV3	0 0%	1 8%	10 83%	4 33%	83	86
	DENV4	0 0%	1 8%	10 83%	4 33%	33	30
EP20/E	DENV1	12 100%	0 0%	0 0%	0 0%	100	100
	DENV2	0 0%	8 67%	0 0%	1 8%	67	97.2
	DENV3	2 17%	0 0%	11 92%	0 0%	92	94.4
	DENV4	0 0%	0 0%	0 0%	10 83%	83	100
EP21/E	DENV1	2 17%	0 0%	0 0%	0 0%	17	100
	DENV2	0 0%	0 0%	0 0%	0 0%	0	100
	DENV3	0 0%	0 0%	0 0%	0 0%	0	100
	DENV4	0 0%	0 0%	0 0%	0 0%	0	100
EP22-23/E	DENV1	4 33%	0 0%	3 25%	0 0%	33	91.6
	DENV2	0 0%	6 50%	4 33%	1 8%	50	86
	DENV3	1 8%	0 0%	10 83%	2 17%	83	91.6
	DENV4	0 0%	1 8%	1 8%	8 67%	67	94.4
EP25/E	DENV1	10 83%	5 42%	12 100%	1 8%	83	50
	DENV2	0 0%	7 58%	1 8%	8 67%	58	75
	DENV3	2 17%	9 75%	12 100%	2 17%	100	63.8
	DENV4	9 75%	4 33%	3 25%	12 100%	100	55.5
EP30/E (first half of the epitope)	DENV1	5 42%	2 17%	7 58%	1 8%	47	72.2
	DENV2	12 100%	12 100%	12 100%	12 100%	100	0
	DENV3	12 100%	12 100%	12 100%	12 100%	100	0
	DENV4	12 100%	11 92%	10 83%	12 100%	100	8.3
EP30/E (latter half of the epitope)	DENV1	0 0%	0 0%	0 0%	0 0%	0	100
	DENV2	0 0%	0 0%	0 0%	0 0%	0	100
	DENV3	0 0%	0 0%	0 0%	0 0%	0	100
	DENV4	0 0%	0 0%	0 0%	0 0%	0	100

Number of sera samples giving positive responses out of 12 sera samples tested per serotype (DENV1, DENV2, DENV3, DENV4), and its percentage (%) are shown. The corresponding epitope/peptide sequences from cross serotypes are based on the peptide arrays; DENV1 (Singapore/S275/1990 (NR-4551), DENV2 (New Guinea C (NR-507), DENV3 (Sleman/1978 (NR-511)), DENV4 (Dominica/814669/1981 (NR-512)

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