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Fig. 1 | BMC Immunology

Fig. 1

From: Repression of the expression of proinflammatory genes by mitochondrial transcription factor A is linked to its alternative splicing regulation in human lung epithelial cells

Fig. 1

TFAM selectively represses gene expression in human lung epithelial A549 cells. a Enforced expression of TFAM in A549 cells detected by western blot experiment, gels and blots were cropped. b Heat map display of the hierarchically clustered Pearson’s correlation matrix resulting from comparing the expression level of each gene in the control and TFAM-overexpression transcriptomes. c Volcano plot shows the expression change of genes between the control and TFAM-overexpression transcriptomes, leading to the identified DEGs. Upregulated genes (FC ≥ 2; FDR < 0.05) are labeled in red and downregulated genes (FC ≤  − 2; FDR < 0.05) are labeled in blue. d Hierarchical clustering of the expression levels of all the identified DEGs in control and TFAM-overexpression samples. FPKM values are log2-transformed and then median-centered by each gene. e The top 10 GO biological processes of TFAM-up and down-regulated genes. f The top 10 KEGG pathways of TFAM-up and down-regulated genes. g Hierarchical clustering of the expression levels ISGs in the control and TFAM-overexpression A549 cells; these ISGs were negatively regulated in the Tfam-deficiency mouse murine embryonic fibroblast (MEF) cells [47]

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