Fig. 4

Validation of TFAM-regulated RASEs. The RNA-seq read distribution profiles of the RASEs subjected to RT-qPCR were depicted on the left, with the read density map and the numbers of alternative and model junction reads being shown. The black lines represent the splice junctions composing ASEs. The schematic diagrams depict the structures of ASEs are shown on the right upper panel, which are similar as in Fig. 3B. The results of the RNA-seq and RT-qPCR quantification of the change of the ratio between the alternative and model splicing products are shown on the right lower panel. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01