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Fig. 1 | BMC Immunology

Fig. 1

From: Immunogenicity profile in African green monkeys of a vaccine candidate based on a mutated form of human Interleukin-15

Fig. 1

Characterization of purified IL-15 D8SQ108S. a SDS-PAGE and b Western blot analysis of purified IL-15 D8SQ108S. Five hundred microliters of the main peak collected from the RP chromatography were concentrated, then 5 µg of purified IL-15 D8SQ108S (lane 2) were loaded onto a 15% SDS-PAGE gel. Lane 1: protein molecular weight marker (12.5–97.4 kDa). The anti-huIL-15 monoclonal antibody MAB 9 was used to detect the protein of interest. Lane 1: 5 µg of purified IL-15 D8SQ108S. Lane 2: 5 µg of commercial huIL-15 (positive control). c Determination of IL-15 D8SQ108S purity by RP-HPLC. Fifty micrograms of the purified protein were injected into a C8 column, obtaining a principal peak with 98% purity

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BMC Immunology

ISSN: 1471-2172

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