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Fig. 3 | BMC Immunology

Fig. 3

From: Immunogenicity profile in African green monkeys of a vaccine candidate based on a mutated form of human Interleukin-15

Fig. 3

IL-15-specific antibody response elicited in monkeys immunized with IL-15 D8SQ108S or mhIL-15. The ELISA plate was coated with 1 µg/ml of IL-15 D8SQ108S or mhIL-15 and the serum from each animal was evaluated in twofold serial dilutions (starting dilution 1:4000). All sera tested were collected before beginning the scheme (pre-immune) and 15 days after the second and third immunizations. a Serum dilution curves of a representative animal per experimental group. Data represent the mean values of OD450nm ± standard deviation (SD) from two independent experiments performed in triplicate. Dashed line represents the value interpolated in the serum dilution curves, which corresponds twice the mean value of pre-immune serum OD450nm from the representative animal of each group. b IgG antibody titers against IL-15 in immunized monkeys. The individual antibody titers, expressed as 1/dilution, are represented. The lines represent the median values with interquartile range of antibody titers calculated from duplicate samples of individual monkeys (n = 3) corresponding to each experimental group (200 µg mhIL-15, 200 µg IL-15 D8SQ108S and 350 µg IL-15 D8SQ108S)

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