Fig. 5
Effect of immune sera on the CTLL-2 cells proliferation induced by huIL-15 and siIL-15. a CTLL-2 cells (5 × 103 cells/well) were cultured for 72 h with 300 pg/mL of huIL-15 and serial dilutions 1:2 (initial dilution 1:100) of the pool of sera collected 15 days after the second and third immunizations from animals immunized with 200 µg of mhIL-15, 200 µg of IL-15 D8SQ108S or 350 µg of IL-15 D8SQ108S. b CTLL-2 cells (5 × 103 cells/well) were cultured for 72 h with 300 pg/mL of siIL-15 and serial dilutions 1:2 (initial dilution 1:25) of the pool of sera collected 15 days after the third immunization from animals immunized with the same antigen doses mentioned above. Cell proliferation was measured by MTT staining. Data represent the mean values of OD578nm ± standard deviation (SD) from two independent experiments performed in triplicate. Dashed lines represent the minimum and maximum proliferation of the cells