Fig. 1
From: Determining if T cell antigens are naturally processed and presented on HLA class I molecules
Schematic overview of determining antigen-specific T cell responses using electroporated B cells electroporated with full-length mRNA. a pcRNA2SL vector engineered to express a gene of interest is linearized through restriction digestion; b linearized expression plasmid is transcribed into full length 5′ capped and 3′ polyA tailed mRNA through in vitro transcription with a commercially available kit; c peripheral blood B cells are isolated from patient material through magnetic selection and full-length mRNA is introduced via electroporation; d peripheral blood or tumor infiltrating T cells are co-incubated with autologous B cells loaded with full-length mRNA and antigen-specific responses are detected. R.E. restriction element, TIL tumor infiltrating lymphocytes