Fig. 2
From: Determining if T cell antigens are naturally processed and presented on HLA class I molecules
B cells are readily expanded in culture and electroporated with full-length mRNA. a Cell counts of magnetically isolated B cells co-cultured with NIH3T3-CD40L feeder cells and IL-4, error bars represent standard deviation of three independent experiments; b phase contrast and immunofluorescent photomicrographs of ex vivo expanded B cells electroporated without or with mRNA encoding GFP. Images acquired 2 h after electroporation; c flow cytometric analysis of ex vivo expanded B cells electroporated with mRNA encoding GFP for fluorescent signal; d viability of B cells electroporated without mRNA (control), with GFP mRNA or with HPV16 mRNA was assessed via sytox staining on flow cytometry or via acridine orange/propidium iodide staining on the automated cell counter. e Flow cytometric analysis of cell surface CD80, CD86 and HLA-DR expression on B cells before and after in vitro expansion, and with or without electroporation. Hashed bars indicate B cells electroporated with no mRNA, GFP mRNA or HPV16 E7 mRNA. ***, p < 0.001