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Fig. 2 | BMC Immunology

Fig. 2

From: ERAP1 is a critical regulator of inflammasome-mediated proinflammatory and ER stress responses

Fig. 2

ERAP1 deficiency enhances cytokine and chemokine responses in macrophages following inflammasome stimulation. Bone marrow-derived macrophages (5 × 105 cells) were plated into 24-well plates. Cells were primed with LPS (20 ng/mL) for 16 h, and then stimulated for another 24 h with various NLR inflammasome agonists, as indicated. Cell supernatants were collected, and a 23-plex multiplex assay was performed. Production of IL-1α (A), G-CSF (B), IL-6 (C), MCP-1 (D), TNFα (E), and IL-10 (F) following NLRs stimulation is shown. Cells were plated in quadruplicate. Data are expressed as means ± SEM. These figures are representative of four independent experiments

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