Fig. 6

The effects of DHA feeding on the mRNA expression of inflammatory mediators at day 3 and day 7 post-MCMV infection. A mRNA levels of IFN-α and IFN-β in the peripheral blood cells of both control and DHA-fed mice following MCMV infection at 12, 24, 48 and 72 h determined by RT–qPCR. B, C mRNA levels of the cytokines and chemokines, including IFN-α, IFN-β, IL-4, IL-6, IL-10, IL-12, TNF-α, CCL-2 and CCL3, in the spleen of both control and DHA-fed mice at days 3 (B) and 7 (C) postinfection were determined by RT–qPCR. Each symbol represents an individual mouse, and the blue dots and red square represent control and DHA-enriched diet-fed mice, respectively. For each experiment, n = 5 to 9 pooled from 3 independent experiments (A–C). Error bars represent interquartile ranges. *p < 0.05; **p < 0.01