Fig. 7From: Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopyThe addition of anti-CCL2 to 5-FU shows a change in hypoxia. A Hypoxia quantification through IHC quantification using CA-IX (10X objective, 0.3NA, scale bars are 50 μm). B The average hypoxic area was calculated per FOV (n = 27 FOVs per group). A mixed effects model was used to calculate statistical differences. Plot created in Prism (GraphPad)Back to article page