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Table 2 The results of LDH cytotoxicity assay in each donor and controls against human lymphoblastoid cell line pulsed with BKPyV PepMixes

From: In Vitro Generation of BK polyomavirus-specific T cells for adoptive cell therapy in refractory cystitis hemorrhagic patients after hematopoietic stem cell transplantation

 

T cell + BKPyV PepMix-pulsed mo-DC + IL-2

Controls

E/T ratio

Donor 1 (%)

Donor 2 (%)

Donor 3 (%)

T cell + Actin-pulsed mo-DC + IL-2 (%)

T cell + IL-2 (%)

20:1

57.16 ± 4.83

59.16 ± 4.11

52.86 ± 4.71

23.56 ± 5.34

19.34 ± 5.10

10:1

38.53 ± 4.22

36.70 ± 4.33

34.06 ± 3.72

16.36 ± 3.48

12.56 ± 4.07

5:1

22.13 ± 1.95

21.01 ± 3.45

19.26 ± 1.40

9.10 ± 2.15

8.50 ± 2.47

1:1

10.33 ± 1.02

9.90 ± 0.80

11.10 ± 2.5

3.46 ± 1.13

3.07 ± 1.64