Fig. 3

Effects of long-term cryopreservation on PBMCs subtypes. A The proportions of leucocytes. B The proportions of CD14⁺ Monocytes in CD45⁺ gate. C The proportions of CD16⁺CD56⁺ NK in CD45⁺ gate. D The proportions CD19⁺ B in CD45⁺ gate. E The proportions CD3⁺ T in CD45⁺ gate. F The proportions CD3⁺CD16⁺CD56⁺ NKT in CD45⁺ gate. The tested samples are fresh isolated and cryopreserved(1, 3 and 6 mo, respectively) PBMCs. G The proportions of total ILC and the subtypes ILC1,ILC2,ILC3 in Lineage⁻lymphocytes or H the proportions of the subtypes ILC1,ILC2,ILC3 in total ILC.*P < 0.05, **P < 0.01,***P < 0.001