Fig. 4

Effects of long-term cryopreservation on Tc, Th, apoptosis and proliferation (n = 20, 12, 12 respectively). A The proportion of Th and Tc in total T cells and B the proportion of apoptosis and C proliferation in fresh isolated and cryopreserved (1, 3 and 6 mo, respectively) PBMCs. D The proliferation results of flow cytometry in fresh isolated and cryopreserved (1, 3 and 6 mo, respectively) PBMCs.*P < 0.05, **P < 0.01,***P < 0.001