Table 1 The effect of fresh isolation and cryopreservation on innate and adaptive immune cells
| Â | Fresh (%) | 1 m (%) | Â | 3 m (%) | Â | 6 m (%) | Â |
|---|---|---|---|---|---|---|---|
Mean (range) | Mean (range) | Fresh versus 1 m P | Mean (range) | Fresh versus 3 m P | Mean (range) | Fresh versus 6 m P | |
Lymphocytes | 86.6 (72.2–94.9) | 87.8 (80–94.6) | ns | 87.5 (79.3–94.1) | ns | 89.3 (81.9–95.7) | ns |
T | 73.9 (59.4–80.7) | 82.2 (67.1–89.5) |  < 0.0001 | 81.3 (68.2–87.6) |  < 0.0001 | 81.0 (67.3–88.6) |  < 0.0001 |
B | 5.77 (2.5–7.3) | 4.56 (2.4–7.0) | 0.0004 | 5.51 (3.0–7.7) | ns | 4.19 (1.7–7.9) | 0.0004 |
NK | 12.3 (6.8–24.6) | 10.0 (4.1–23.0) | 0.0136 | 7.6 (3.2–19.0) |  < 0.0001 | 8.9 (3.9–18.7) |  < 0.0001 |
NKT | 1.7 (0.1–9.3) | 1.8 (0.1–10) | ns | 1.3 (0.1–10.3) | ns | 1.6 (0.1–12) | ns |
Monocyte | 3.9 (0.5–12.4) | 0.2 (0.0–0.8) | 0.0004 | 0.6 (0.0–1.9) | 0.0008 | 0.2 (0.0–0.8) | 0.0004 |
ILC | 44.1 (29.9–60.2) | 19.1 (11.8–28.6) | 0.0002 | 16.6 (11.6–30.1) | 0.0004 | 31.1 (13.6–55.6) | ns |
ILC1 | 36.0 (25.1–54.6) | 14.4 (9.4–21.4) | 0.0013 | 10.3 (6.2–21.1) | 0.0012 | 28.0 (11.1–53.0) | ns |
ILC2 | 4.3 (1.8–8.1) | 1.7 (0.7–3.7) | 0.0176 | 1.7 (0.9–2.9) | 0.0148 | 1.6 (0.8–2.1) | 0.0308 |
ILC3 | 3.9 (1.7–6.3) | 3.0 (1.4–5.6) | ns | 4.5 (2.1–6.1) | ns | 1.5 (1.0–2.2) | 0.0088 |