Comprehensive evaluation of the effects of long-term cryopreservation on peripheral blood mononuclear cells using flow cytometry

Table 2 Effect of long-term cryopreservation on different stage of T cells

	Fresh (%)	1 m (%)		3 m (%)			6 m (%)
	Mean (range)	Mean (range)	Fresh versus 1 m P	Mean (range)	Fresh versus 3 m P	1 m versus 3 m P	Mean (range)	Fresh versus 6 m P	1 m versus 6 m P	3 m versus 6 m P
CD4
Naïve	31.9 (4.9–66.5)	30.1 (12.8–63.4)	ns	31.6 (15.0–48.4)	ns	ns	14.8 (5.4–40.5)	< 0.0001	< 0.0001	< 0.0001
Central memory	41.0 (22.5–54.5)	44.3 (24.3–58.6)	ns	34.1 (24.1–42.3)	0.0013	< 0.0001	44.0 (27.2–58.7)	ns	ns	< 0.0001
Effector	4.8 (2.5–11.4)	4.8 (2.4–9.7)	ns	2.9 (0.9–8.8)	< 0.0001	< 0.0001	3.8 (0.8–14.8)	0.0264	ns	ns
Effector memory	22.4 (7.2–38.5)	20.2 (8.2–30.4)	ns	31.6 (15.7–50.0)	< 0.0001	< 0.0001	72.4 (14.1–58.3)	< 0.0001	< 0.0001	0.0014
Actived	1.5 (0.3–3.9)	2.1 (0.6–6.1)	ns	1.4 (0.6–2.5)	ns	0.0467	1.8 (1.0–3.7)	ns	ns	ns
CD8
Naïve	27.1 (5.4–52.6)	31.3 (13.1–50.9)	0.0449	26.7 (7.1–47.9)	ns	0.0003	16.6 (3.7–46.7)	< 0.0001	< 0.0001	< 0.0001
Central memory	6.5 (1.9–12.5)	8.1 (2.3–13.6)	0.0178	4.7 (1.3–8.0)	0.0073	0.0001	6.2 (1.7–14.0)	ns	ns	0.0091
Effector	41.0 (25.6–74.6)	37.9 (21.6–63.2)	0.0065	39.1 (22.8–70.1)	ns	ns	45.1 (24.2–79.3)	0.0053	< 0.0001	< 0.0001
Effector memory	25.3 (25.6–58.2)	23.2 (21.6–63.2)	ns	29.5 (22.8–70.1)	0.0024	< 0.0001	32.1 (24.4–79.3)	< 0.0001	< 0.0001	ns
Actived	2.0 (0.5–4.7)	1.9 (0.8–4.3)	ns	1.7 (0.4–4.2)	ns	ns	0.5 (0.1–2.2)	< 0.0001	< 0.0001	< 0.0001

ISSN: 1471-2172