Fig. 2

TFRs function and cytokine production profile. A Schematic of experimental design for co-culture assays. To increase cell numbers for the assay lymph node mononuclear cells (LMCs) were sorted for naïve B cells and enriched for TFRs (CD4⁺CD25⁻CD127⁺) and TFH cells (CD4⁺CD25⁺CD127). The TFH and naïve B cell cells were co-cultured for 8 days in presence or absence TFRs. As negative control B cells were cultured alone. IgG in the supernatant was detected by an ELISA. B Summary plot of IgG antibody detection by ELISA. C mRNA expression of IL-10 detected by ddPCR in HIV negative (n = 4), HIV-infected treated (n = 8) and HIV-infected untreated (n = 5) subjects. P values were determined using Mann–Whitney U test