Fig. 7

FCRL3 and DPP4 define distinct TFR populations. A Flow cytometry plot showing co-expression of FCRL3 and DPP4 by TFRs. B Representative contour plots demonstrating overlay of TFR populations (grey) and cytokine secreting cells (red) and summary plots showing C IL-2, D TNF-α and E IFN-γ expressing cells within TFR subsets. F Representative flow cytometry plots and G aggregate data depicting co-expression of IL-2 and TNF-α by the TFR subpopulations. H Representative flow plot demonstrating overlay of FCRL3⁺ TFRs (grey) populations and FOXP3⁺(red) cells together with DPP4⁺Tfrs (grey) and FOXP3⁺ cells (red). I Representative image of immunoflouresently stained LN sections with zoomed-in images below, LNs were stained with BCL-6 (green), FOXP3 (red) and DPP4 (white)