Fig. 3
From: Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer
Effect of GW4869 on the secretion of exosomes and number of CD206+ macrophages. A Effect of GW4869 on AChE activity. After PCa cells were treated with GW4869 for 48 h, CM from PC-3-M-2B4 or PC-3-M-1E8 was collected, and then the AChE activity in the above CM was detected by ELISA. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. B Effect of GW4869 on the release of PCa exosomes. After GW4869 treatment, the amount of exosomes in the CM from PC-3-M-2B4 or PC-3-M-1E8 cells was evaluated using flow cytometry. C CM from PCa cells treated with GW4869 reduced the CD206 expression in macrophages. After macrophages were co-cultured with CM-2B4-GW4869 or CM-1E8-GW4869, respectively, we determined the CD206 expression of subtypes of macrophages with an anti-CD-206-FITC antibody (green)