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Fig. 2 | BMC Immunology

Fig. 2

From: NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

Fig. 2

LPS/ATP stimulation activates NLRP3/caspase-1/IL-1β axis and promotes MHC-I expression. A Western blot analysis showed increased expression of NLRP3, pro-caspase-1, cleaved caspase-1, pro-IL-1β and mature IL-1β in the Raw 264.7 macrophages under LPS/ATP stimulation. B, C ELISA analysis showed that the expression of IL-1β and TNF-α in the Raw 264.7 macrophages were up-regulated by LPS/ATP. D–G ELISA analysis showed elevated levels of IFNs and MCP-1 produced by the stimulated macrophages. H The mRNA expression of MHC-I in the co-cultured C2C12 cells was increased under LPS/ATP stimulation and peaked at 48 h. I, J The protein expression of MHC-I in the co-cultured C2C12 cells was increased under LPS/ATP stimulation and peaked at 72 h. *p < 0.01, **p < 0.001, compared with control group or compared between the groups connecting with the line

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BMC Immunology

ISSN: 1471-2172

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