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Fig. 3 | BMC Immunology

Fig. 3

From: NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

Fig. 3

Genetic knockdown of NLRP3 suppresses MHC-I overexpression in C2C12 cells. A Raw 264.7 macrophages were transfected with siRNA targeting NLRP3, and the transfection efficiency was measured by western blot analysis. B, C Raw 264.7 macrophages pretreated with NLRP3-siRNA were stimulated with 200 ng/ml LPS + 500 μM ATP, and then the expression of MHC-I in the co-cultured C2C12 cells was significantly reduced compared with those pretreated with NC-siRNA, as revealed by western blot. D, E The stimulated Raw 264.7 macrophages were pretreated with NLRP3-siRNA or NC-siRNA, and the co-cultured C2C12 cells were added with neutralizing IL-1β monoclonal antibody. No differences in the expression of MHC-I in the co-cultured C2C12 cells were found between those groups stimulated with 200 ng/ml LPS + 500 μM ATP or with PBS, as showed by western blot. **p < 0.001, compared with control group; ¶¶p < 0.001, compared with before treatment

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BMC Immunology

ISSN: 1471-2172

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