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Fig. 5 | BMC Immunology

Fig. 5

From: NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

Fig. 5

IL-1β is crucial for MHC-I up-regulation in C2C12 cells. A, B Recombinant IL-1β was added into the C2C12 cells. The expression of MHC-I in C2C12 cells peaked when treated by 1 ng/ml IL-1β for 48 h (n = 5). C Raw 264.7 macrophages were transfected with siRNA targeting IL-1β, and the transfection efficiency was measured by western blot analysis. D, E LPS/ATP stimulated Raw 264.7 macrophages were pretreated with IL-1β-siRNA or NC-siRNA, and the MHC-I expression in the co-cultured C2C12 cells was significantly lower with the former than with the latter pretreatment, as analyzed by western blot. *p < 0.01, **p < 0.001, compared with control group; ¶¶p < 0.001, compared with before treatment

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BMC Immunology

ISSN: 1471-2172

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