Transition of tumor-associated macrophages from MHC class IIhi to MHC class IIlow mediates tumor progression in mice
© Wang et al; licensee BioMed Central Ltd. 2011
Received: 7 May 2011
Accepted: 4 August 2011
Published: 4 August 2011
Tumor-associated macrophages (TAMs) are the most abundant immune cells within the tumor stroma and play a crucial role in tumor development. Although clinical investigations indicate that high levels of macrophage (MΦ) infiltration into tumors are associated with a poor prognosis, the exact role played by TAMs during tumor development remains unclear. The present study aimed to investigate dynamic changes in TAM major histocompatibility complex (MHC) class II expression levels and to assess the effects of these changes on tumor progression.
Significant inhibition of tumor growth in the murine hepatocellular carcinoma Hepa1-6 model was closely associated with partial TAM depletion. Strikingly, two distinct TAM subsets were found to coexist within the tumor microenvironment during Hepa1-6 tumor development. An MHC class IIhi TAM population appeared during the early phase of tumor development and was associated with tumor suppression; however, an MHC class IIlow TAM population became increasingly predominant as the tumor progressed.
Tumor progression was positively correlated with increasing infiltration of the tumor tissues by MHC class IIlow TAMs. Thus, targeting the transition of MΦ may be a novel strategy for drug development and immunotherapy.
Macrophages (MΦ) represent the most abundant immune cell population in the tumor microenvironment and play a key role in tumor development [1, 2]. High levels of MΦ infiltration into tumor tissues are associated with a poor prognosis; this is particularly true for hepatocellular carcinoma (HCC) [3–6]. Although a decreased number of macrophages correlates with a reduction in tumor growth in several tumor graft models [7, 8], there are some exceptions. For example, depletion of Kupffer cells worsens the prognosis of tumor-bearing mice in peritoneal xenograft models because the cancer cells are able to metastasize to the liver; thus, the mice die from the increased tumor burden in the absence of MΦ [9, 10]. These contradictory reports highlight the fact that little is known about the exact role of tumor-associated macrophages (TAMs) during tumor development.
MΦ are a highly heterogeneous cell population. This is because their phenotypes and diverse functions are shaped by the tumor microenvironment . MΦ can be classified on the basis of two distinct activation states. Classically activated MΦ (M1), induced by IFN-γ or microbial products, produce high levels of proinflammatory cytokines (IL-12 and IL-23), express major histocompatibility complex (MHC) molecules and iNOS, and act as the primary source of anti-tumor immune cells [12–14]. In contrast, alternatively activated MΦ (M2), polarized by IL-4 or/and IL-13, secrete anti-inflammatory cytokines and are characterized by increased arginase-1 activity and the expression of Ym-1, MGL, Fizz1, and MSF [15–17]. Functionally, M2 MΦ are thought to suppress inflammation and to facilitate wound healing by promoting angiogenesis and tissue remodeling [15, 18]. A recent study shows that mouse mammary tumors contain phenotypically and functionally distinct TAM subsets, and that these subsets promote tumor growth via different mechanisms . HLA-DRhigh and HLA-DRlow IL-10+ monocytes/MΦ have also been identified in different regions within tumor tissues, where they mediate T cell anergy through PD-L1 and increase tumor cell migration and invasion [3–5, 20]. However, the precise role played by these heterogeneous TAM subsets in tumor progression has rarely been reported.
Clodronate (Cl2MDP)-encapsulated liposomes cause irreversible damage to MΦ in vivo, thereby efficiently decreasing the number of infiltrating MΦ [21, 22]. Strategies incorporating liposome-mediated MΦ depletion have been successfully used in murine tumor models of teratocarcinoma, rhabdomyosarcoma, lung cancer, and melanoma [7, 23, 24].
In the present study, Cl2MDP-liposomes were used to partially deplete TAMs in a murine transplanted hepatoma model to investigate the exact role played by TAMs during tumor development and the mechanisms underlying TAM-mediated tumor progression.
Partial depletion of MΦ using Cl2MDP-liposomes
Partial depletion of MΦ inhibits tumor progression in tumor-bearing mice
Tumor progression correlates with the level of TAM infiltration
TAMs in tumor-bearing mice are a heterogeneous population
To determine the effects of Cl2MDP-liposomes on the two TAM subsets, we examined expression of MHC class II in both untreated and TAM-depleted tumors using immunohistochemistry and flow cytometry. As shown in Figure 4F, there were differences in the expression of MHC class II between untreated and TAM-depleted tumors. Although the total number of TAMs expressing MHC class II decreased after treatment with Cl2MDP-liposomes, the number of MHC class IIlow TAMs in the treated tumors was significantly lower than in untreated tumors. In contrast, the number of MHC class IIhi TAMs was not significantly affected by treatment with Cl2MDP-liposomes (Figure 4G and 4H). Taken in the context of the data presented in Figure 3A, these results suggest that after depleting the majority of TAMs, the remaining MΦ undergo transition from MHC class IIhi to MHC class IIlow at an accelerated rate, resulting in delayed tumor growth.
Taken together, these data demonstrate that two TAM subsets exist within the tumor tissues and that transition between the two sub-populations is closely related to tumor progression, during which the predominant MHC class IIhi subset may shift to an MHC class IIlow subset.
MHC class IIlow TAMs promote tumor growth and MHC class IIhi TAMs promote tumor inhibition
MHC class IIlow TAMs inhibit T cell activation and promote invasion of tumor cells by secreting immunosuppressive factors
We also examined the expression of MMP-9 and VEGF in both subsets using RT-PCR and western blotting. The expression of MMP-9 and VEGF in MHC class IIhi TAMs was lower than that in MHC class IIlow TAMs (Figure 6D and 6E). To assess the effects of the different TAM subsets on the invasiveness and metastasis of mouse hepatoma cells, an invasion assay was performed using HCC cells. The invasiveness of cancer cells was markedly enhanced by MHC class IIlow TAMs (p < 0.01; Figure 6F and 6G). This result suggests that MHC class IIlow TAMs promote cell invasion to a greater extent than MHC class IIhi TAMs.
This study showed that partial depletion of MΦ by Cl2MDP-liposomes efficiently modulates the tumor microenvironment and tumor progression in the Hepa1-6 cell-transplanted tumor model. Furthermore, heterogeneous TAM populations were found to coexist in the tumor microenvironment. MHC class IIhi TAMs appeared during the early phases of tumor development and showed tumor suppressive activity. In contrast, MHC class IIlow TAMs became more dominant as the tumors progressed, and were alternatively (rather than classically) activated and promoted tumor growth.
Cl2MDP is a bisphosphonate developed to treat osteolytic bone disease . The free drug does not easily enter cells and has an extremely short half-life in the circulation and body fluids. However, it is ingested specifically by MΦ and has a longer half-life when encapsulated in liposomes [21, 22]. MΦ are irreversibly damaged owing to intracellular accumulation of the drug and undergo apoptosis because of the formation of toxic ATP analogues [21, 22, 25]. This strategy efficiently reduces the number of MΦ in vivo and has been used successfully to investigate the role of MΦ in animals [7, 23, 24, 26, 27]. The results of the present study show that treatment with Cl2MDP-liposomes efficiently and specifically reduces the number of TAMs in a murine transplanted hepatoma model. Although tumor regression following TAM depletion has been reported in a human hepatoma xenograft nude mouse model treated with sorafenib, treatment with Cl2MDP-liposomes alone has no inhibitory effect on tumor growth in tumor-bearing nude mice . This disparity can be explained by the absence of T cell-mediated cellular immune responses in nude mice. Another possible explanation is that the effects of Cl2MDP-liposome treatment are limited in nude mice. That said, the role of MΦ in tumor-bearing mice and rats is still extensively studied using Cl2MDP-liposomes.
The choice of administration route used for liposome treatment enables some degree of selectivity with respect to the MΦ populations targeted . Injection of Cl2MDP-liposomes via the i.v. route depletes MΦ in liver, spleen, bone, and circulation, whereas s.c. injection only affects MΦ in the lymph nodes . A recent study shows that intratumoral (i.t.) injection of Cl2MDP-liposomes into tumor-bearing mice significantly delays tumor growth . Although the total number of MΦ affected by i.p. injection is higher than that affected by other routes (e.g. the i.v. route), it necessitates the use of a relatively large volume of Cl2MDP-liposomes . Thus, in the present study, we injected Cl2MDP-liposomes via the i.v. route for orthotopically implanted tumors, and via both i.v. and i.t. routes in the s.c.-implanted tumor model, to maximally reduce the number of TAMs infiltrating the tumors and to minimize any possible toxic side effects mediated by the Cl2MDP-liposomes. We did not examine the effect of i.v. or i.t. injection of Cl2MDP-liposomes on s.c.-transplanted tumors, as the combination of i.v. injection plus i.t. administration was thought to be the effective procedure for depleting TAMs in our models. However, the choice of injection route needs to be validated by additional investigations.
Unlike lymphocytes, MΦ do not express specific markers, although F4/80, CD68, and CD11b are commonly used to label these cells. A rat monoclonal anti-MΦ antibody [RM0029-11H3] was developed against isolated mouse MΦ and was used to label MΦ in some studies [30, 31]. A recent study shows that MΦ can be classified into CD11b+Ly6Chi and CD11b+Ly6Clow populations based on their expression of CD11b and Ly6C . Differences in the expression levels of these biomarkers can be used to examine MΦ subpopulations. In the present study, histological analyses showed that TAMs could be exclusively labeled using antibodies to F4/80, CD68, and with the rat monoclonal anti-MΦ antibody [RM0029-11H3], although there were quantitative differences in the expression levels of these biomarkers.
MΦ within tumors are polarized toward the M2 phenotype by tumor-derived factors, which cause them to promote tumor progression [3, 4, 14, 32–34]. IL-10 and TGF-β play an important role in promoting tumor growth. IL-10 is likely to induce TAM-derived CCL18, which is a chemoattractant for naive T cells, followed by T cell anergy via altered STAT1 expression [35–37]. The results of the present study showed that MHC class IIlow TAMs expressed high levels of IL-10 and TGF-β. The role of these cytokines was verified, at least in part, by blocking cytokine-mediated T cell suppression in vitro using anti-IL-10 and anti-TGF-β mAbs. Interestingly, the levels of IL-10 and TGF-β, which are expressed by MHC class IIlow TAMs in the TS/A tumor model, are low . This may be explained by disparities in the experimental models; for example, the constitution of the tumor microenvironment may be different. There may also be a more complex mechanism that mediates suppression of T-cell polyclonal activation by MHC class IIlow TAMs in the TS/A tumor model. However, MHC class IIlow TAMs in the Hepa1-6 tumor model are more likely to mediate suppression of T-cell activation via production of IL-10 and TGF-β. Although the exact mechanisms underlying MHC class IIlow TAM-mediated suppression of tumor growth in vivo are yet to be elucidated, our observations provide an insight into how TAMs subvert anti-tumor adaptive responses and redirect T cell activity to favor tumor growth. TAM-mediated T cell anergy may play an important role in tumor promotion. Our investigation also shows that MHC class IIlow TAMs promote invasion of Hepa1-6 cells via the production of angiogenesis-associated molecules such as VEGF and MMP-9. This suggests that MHC class IIlow TAM subset-mediated angiogenesis could be another important cause of runway tumor progression. Thus, the mechanisms underlying TAM subset-mediated tumor growth are intricate and need further study.
TAMs are a heterogeneous population that may have both inhibitory and stimulatory influences on malignant growth . The transition between complex phenotypes depends on the tumor milieu [19, 39]. For example, studies in patients with HCC reveal that two distinct populations (HLA-DRhigh and HLA-DRlow IL-10+) of monocytes/MΦ exist within different regions of the tumor, which modulate T cell activation via different mechanisms [4, 5]. Although the MHC class IIhi TAM and MHC class IIlow TAM populations in the TS/A tumor model mediate tumor progression through different mechanisms, dynamic analysis of TAM subsets shows that there is a balance between these populations . This is consistent with our observation that a shift from MHC class IIhi to MHC class IIlow in the Hepa1-6 tumor model correlates with tumor progression. Infiltrating monocytes are more likely to differentiate into MHC class IIhi TAMs during the early phase of tumor development, whereas MHC class IIlow TAMs become gradually more prominent as the tumors progress. However, this study also showed that MHC class IIlow TAMs promoted tumor progression, whereas MHC class IIhi TAMs had a suppressive effect on tumor growth. Further investigation into the effects of the tumor microenvironment on the balance between TAM subsets may help to explain this discrepancy.
This study shows that TAM depletion causes tumor regression in a murine hepatoma xenograft mouse model. Heterogeneous TAM subsets co-exist within the tumor microenvironment, and there is a transition from MHC class IIhi to MHC class IIlow TAMs that correlates with tumor progression. The MHC class IIhi TAM population appears during the early phase of tumor development and contributes to tumor suppression, whereas the MHC class IIlow population becomes dominant as the tumor progresses. MHC class IIlow TAMs are alternatively activated and promote tumor growth. Therefore, targeting the transition of MΦ may be a novel strategy for drug development and immunotherapy.
The following reagents were used in the study: Rat anti-mouse F4/80 mAb (AbD; Serotec, Kidlington, UK), a rat anti-MΦ mAb [RM0029-11H3] (a rat monoclonal antibody against isolated mouse MΦ; Abcam, Cambridge, MA, USA), rat anti-mouse MHC class II (I-Ab,d,q/I-Ed,k) mAb (BioLegend, San Diego, CA, USA), and rat anti-mouse-IL-10/TGF-β and rabbit-anti-mouse-CD31/VEGF/MMP-9 mAb (Santa Cruz Biotechnology, Santa Cruz, CA, USA). The IL-10 and TGF-β enzyme-linked immunosorbent assay (ELISA) kits were purchased from Bender Medsystems (GmbH, Vienna, Austria). All fluorescently-labeled mAbs and isotype control mAbs were obtained from eBioscience (San Diego, CA, USA). Clodronate (dichloromethylene diphosphonate, Cl2MDP) was a generous gift from Roche Diagnostics (GmbH, Mannheim, Germany). All other reagents were obtained from Sigma. (St Louis, MO, USA) unless otherwise indicated.
Female C57BL/6 and BALB/c mice (6-8 weeks old) were obtained from Beijing Vital River Experimental Animals, Co. Ltd. All animal experiments were approved by the Laboratory Animal Committee of Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Science.
To investigate the effects of Cl2MDP-liposomes on tumor growth, naïve mice were first injected i.v. with 200 μl of C12MDP-liposome suspension 2 days prior to tumor inoculation. Peripheral blood (50 μl) was collected retro-orbitally 0, 24, and 48 h after treatment and immediately transferred to ethylenediaminetetraacetic acid (EDTA) -containing tubes (BD Bioscience). Whole blood cells were stained with APC-conjugated anti-CD11b and FITC-conjugated anti-F4/80 mAbs for 30 min in the dark on ice. The cells were then washed and the red blood cells lysed with FACS lysis buffer (BD Bioscience). The remaining white blood cells were washed again and resuspended in PBS containing 1% fetal calf serum (FCS). Flow cytometry data were acquired using a BD FACSAria and analyzed using FLOWJO software version 7.6.0 (Tree Star, Inc., Ashland, OR, USA). After tumor inoculation, mice were treated with Cl2MDP-liposomes via both i.v. (200 μl) and i.t. (50 μl) routes on Days 3, 8, 13, and 18. PBS-liposomes and saline were used as the controls. Tumor size was measured every 4 days using calipers and calculated using the formula: 0.52 × a × b2 (a: long diameter of the tumor; b: short diameter of the tumor). After 20 days, tumor-bearing mice were sacrificed for further analysis.
For the orthotopic tumor model, 200 μl of Cl2MDP-liposomes were injected i.v. on Day 2 before surgery, and at 3, 8, and 13 days after surgery. After 15 days, mice were sacrificed and the tumors were removed and weighed.
Preparation of Cl2MDP-liposomes
Cl2MDP-liposomes were prepared as described previously [21, 22]. Briefly, a mixture of 8 mg of cholesterol and 86 mg of phosphatidylcholine was prepared in chloroform in a round-bottom flask. The thin film on the interior of the flask after low-vacuum evaporation at 37°C was dissolved in 10 ml of 0.7 M Cl2MDP solution and incubated for 2 h at room temperature under Argon gas protection, followed by 3 min of sonication and another 2 h of incubation at room temperature. The liposomes were washed five times by centrifugation to remove any free drugs. The final pellet was resuspended in 4 ml of sterile PBS. The control liposomes were prepared using PBS. Cl2MDP-liposomes and PBS-liposomes were stored at 4°C for 2 weeks.
Cell culture and tumor models
The murine HCC cell line, Hepa1-6, and the murine MΦ cell line, RAW264.7, were cultured in DMEM supplemented with 10% FCS and 1% penicillin-streptomycin at 37°C. For the s.c. tumor model, 100 μl of serum-free RPMI 1640 containing 2 × 106 Hepa1-6 cells was injected s.c. into the right anterior axillary fossa of naïve mice. A palpable spherical mass emerged after 4 to 6 days.
An orthotopic hepatoma model was created by transplantation of a small tumor mass into the left liver lobe of naïve mice. Briefly, 2 × 106 Hepa1-6 cells was injected s.c. into the right anterior axillary fossa of naïve mice. The mice were sacrificed when the hepatoma grew progressively and the tumor diameter exceeded 10 mm. The tumors were removed and chopped into small pieces (about 1 mm3) using a scalpel and forceps. The small masses were surgically implanted under anesthesia (a small tumor mass/mouse) into one site in the left liver lobe of mice treated with Cl2MDP-liposomes, PBS-liposomes, or saline.
Preparation of histological specimens (from tumors on Day 20 after treatment with Cl2MDP-liposomes or from tumors on Day 10 after adoptive transfer of sorted TAM populations) and immunostaining were carried out as described previously . Antigen retrieval was performed under high pressure in 10 mM EDTA buffer (pH 8.0) before incubation with primary antibodies to F4/80, CD68, or rat monoclonal anti-MΦ [RM0029-11H3] or IL-10, TGF-β, VEGF, MMP-9, CD31 and MHC class II. Brightfield images were then captured and analyzed.
The number of infiltrating MΦ and the CD31-positive microvessel density were counted in 10 random fields at × 400 magnification (a 0.17 mm2 field), and the results were expressed as the mean number per field ± SD (10 fields per tumor, 5 tumors per group).
Isolation of TAMs and splenocytes
Single cells within the tumor tissue were isolated as described previously with some modifications . Briefly, solid tumors were harvested from tumor-bearing mice on Days 7, 14, 21, and 28 after tumor inoculation or harvested under sterile conditions from tumor-bearing mice untreated or treated with Cl2MDP-liposomes. The tumors were chopped into small pieces using scissors and forceps before incubation with a mixture of enzymes dissolved in RPMI 1640 (400 U/ml collagenase type IV, 0.05 mg/ml collagenase type I, 0.025 mg/ml hyaluronidase, 0.01 mg/ml DNase I and 0.2 units/ml soybean trypsin inhibitor) for 30 min at 37°C. Cells were recovered by centrifugation and resuspended in PBS, containing 1% FBS. The debris and red blood cells were removed using the Ficoll density gradient method. F4/80+MHC IIhi and F4/80+MHC IIlow cells were analyzed and sorted by flow cytometry. Briefly, cells were resuspended in PBS supplemented with 1% FCS, and 1 × 107 cells were incubated with 10 μg of FITC-conjugated F4/80 mAb and 0.2 μg of PE-conjugated MHC class II mAb for 30 min on ice. The cells were then washed with PBS supplemented with 1% FCS to remove unbound Abs. The F4/80+MHC IIhi and F4/80+MHC IIlow cell populations were analyzed and sorted using a FACSAria flow cytometer. All data were analyzed using FLOWJO software version 7.6.0. The purity of the cell populations exceeded 90%.
Single cells were also prepared as described above on Day 10 after tumor inoculation. Briefly, 1 × 107 sorted cells were incubated with 2 μg of APC-conjugated F4/80 mAb and 0.2 μg of PE-conjugated MHC class II mAb for 30 min on ice. The cells were then washed with PBS supplemented with 1% FCS. After fixation, permeation, and washing, the cells were stained with 10 μg of FITC-conjugated IL-10 mAb. The unbound antibodies were removed by washing. The expression of IL-10 by F4/80+MHC IIhi and F4/80+MHC IIlow cells was then analyzed.
Naïve BALB/c mice (6-8 weeks old) were sacrificed and the spleens were harvested using syringes and forceps and single cell suspensions obtained. Red blood cells were removed with ACK lysis buffer (0.15 M NH4Cl, 1 mM KHCO3, 0.1 mM EDTA, pH 7.3), and the remaining splenocytes were washed twice by centrifugation. The splenocytes were counted, diluted to the appropriate concentration in RPMI 1640 supplemented with 10% FCS and 1% penicillin-streptomycin for further investigation.
Real-time quantitative reverse transcription PCR (qRT-PCR) and reverse transcription PCR (RT-PCR)
Total RNA from the sorted TAM populations was isolated using Trizol according to the manufacturer's instructions. qRT-PCR to examine the expression of ym1, mgl1/2, fizz1, arg-1, msf, il-10, tgf-β, mmp-9, vegf, ptge2, il-1β, il-6, il-12, and inos was performed using a One Step SYBR® PrimeScript™ RT-PCR kit according to the manufacturer's instructions (Takara, Dalian, China). β2-mG was used as a the reference gene.
Two-step RT-PCR was performed to examine the expression of mmp-9 and vegf in the sorted TAM populations. cDNA was synthesized from 2 ug of total RNA using Moloney murine leukemia virus reverse transcriptase (Takara) after DNase treatment. The PCR conditions were as follows: 94°C for 5 min followed by 30 cycles at 94°C for 30 s, 60°C for 30 s, and 72°C for 40 s, with a final extension at 72°C for 7 min. The β2-mG gene was used as a reference. The PCR products were analyzed by gel electrophoresis.
Western blot analysis
Total proteins were extracted from the sorted TAM populations and electrophoresed on 10% SDS polyacrylamide gels. The proteins were then transferred to polyvinylidene fluoride membranes (Millipore, MA, USA). Non-specific binding was blocked with 5% (wt/vol) nonfat milk in PBS-Tween-20 (0.1% vol/vol). The membranes were then incubated with primary antibodies against MMP-9 and VEGF at 4°C overnight and with the appropriate secondary antibodies at room temperature for 1 h. Immobilon Western HRP Chemiluminescent Substrate (Cell Signaling Technology, MA, USA) was used to detect the specific signals, and the band intensity was then visualized.
Invasion and migration assays
Tumor cell invasion assays were performed using a Transwell apparatus (Corning, NY, USA) with an 8- μm-pore polycarbonate filter membrane. The upper sides of the membranes were pre-coated with Matrigel matrix (BD, NJ, USA) according to the manufacturer's instructions. Sorted TAMs (2 × 105) were pre-seeded in the lower chamber. The upper chamber was seeded with 1 × 104 Hepa1-6 cells and inserted into the lower chamber. After incubation for 24 h, the cells on the interior of the upper chamber were removed by swabbing with cotton swabs. The polycarbonate membranes were fixed in methanol for 20 min and stained with 0.1% crystal violet for 15 min. The number of cells migrating to the underside of the membranes was counted in five randomly selected fields under a microscope. For the migration assay, the same procedure was used as described above but without addition of Matrigel to the top of the upper chamber membrane.
T cell suppression assays
For the T cell suppression assays, 1 × 105 to 2.5 × 104 sorted TAMs were co-cultured with 2 × 105 naive splenocytes (1:2-1:8) and 1 μg/ml anti-CD3. To neutralize IL-10 and TGF-β, 500 ng/ml IL-10 and/or TGF-β mAb were added to the wells containing MHC class IIlow TAMs, followed by BrdU staining 24 h later. T cell proliferation was assessed using a commercial ELISA kit (Roche Diagnostics, GmbH, Mannheim, Germany), according to the manufacturer's instructions.
The co-culture supernatants were collected and stored at -80°C until used. IL-10 and TGF-β levels were measured using commercially available ELISA kits (Bender Medsystems) according to the manufacturer's instructions.
Adoptive transfer of distinct TAM subsets
For the adoptive transfer assays, 2 × 106 sorted F4/80+MHC IIhi and F4/80+MHC IIlow cells were injected, into the tumors of mice 2 days after inoculation of Hepa1-6 cells. PBS was used as the control. Tumor size was measured every 4 days. The mice were sacrificed 10 days after adoptive transfer and the tumors dissected out for further analysis.
Statistical significance was determined using the Student's t test and GraphPad Prism software. Data were expressed as the mean ± SD. A p value < 0.05 was considered significant.
We thank Weifang Hu and Nangzheng Peng for technical assistance, Dr. Nashunbayaer and Dr. Jianlan You for insightful discussion, and Drs. Schuhr, Sonnentag, and Schaefer (Roche Diagnostics, GmbH, Germany) for kindly providing the clodronate used in the study.
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